PNS HomeGro
Big Savings on Mass Quantities of Natural Live Bacterioplankton: Provide superior nutrition, while controlling nutrients and organic wastes, with purple nonsulfur bacteria.
PNS HomeGro™ contains key items required to economically cultivate purple nonsulfur bacteria (PNSB) using commonplace chemicals and implements. This unique kit presents a more eco-friendly alternative to relatively small, pre-grown bottled products as it significantly reduces shipping/packaging waste. Finished culture treats approximately 8,000 gallons!
The live inoculum in each PNS HomeGro™ kit includes three PNSB species: Rhodopseudomonas palustris, Rhodospirillum rubrum and Rhodobacter sphaeroides.
DIRECTIONS
In addition to sterilizing all implements as described below, wash hands/gloves thoroughly with antibacterial soap and apply a hand sanitizer. Avoid working in drafty and/or dusty areas.
Pour 1 cap household bleach into culture vessel (use vessel cap). Pour 1 cup hot tap water into vessel. Recap vessel and shake. Uncap vessel and completely pour out bleach water, shaking out last drops (do not rinse). Recap vessel immediately without touching inside of cap.
Bring 3 gallons purified water to boil in pot. Sterilize implements (stirring spoon, kitchen thermometer and funnel) with hydrogen peroxide (preferably by spraying) and place on a sterile surface. Add growth medium powder and stir well. Cut heat and cool pot to 90°C/194°F (with pot lid on). Carefully pour into culture vessel through funnel (keep cap on sterile surface); in many instances, for safety, it is best to pour from a pitcher (sterilized as above) rather than directly from the pot. Recap vessel immediately without touching inside of cap.
Cool vessel to room temperature. Remove cap and carefully pour entire contents of inoculum bottle into vessel. Recap with spigot (sterilized as above). Place vessel under continuous, moderate "warm white" light. Maintain incubation temperature of around 29°C/84°F.
Allow approximately 30 days incubation. As reddish sediment accumulates on the vessel floor, shake vessel often to accelerate final growth spurt. Culture may be deemed mature when no further growth has occurred for 5 days or more (typically at around one month). Shake vessel just before each use.
CONTAINS
Culture vessel, spigot, sticker thermometer, growth medium, inoculum (select blend of probiotic cultures featuring Rhodopseudomonas palustris, Rhodospirillum rubrum and Rhodobacter sphaeroides in a proprietary growth medium), instruction sheet.
Requires kitchen funnel, kitchen thermometer, unscented household bleach, hydrogen peroxide (3% or higher), 60W-65W incandescent lamp or equivalent; may require heat pad or similar heat source depending upon cultivation environment temperature. This product is made for aquarium use only; not for human consumption!
Includes exclusively licensed strains. No live cultures made with this product (nor any derivative thereof) may be sold, traded or used for any commercial purpose whatsoever
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